It has been reported that Native Americans have been using jojoba as an alternative therapeutic agent for colds, warts, sore throat, and wounds [25]. Many previous studies reported the antifeedant, insecticidal, and antifungal activities of jojoba [14].

Plant extracts in general and jojoba oil extracts in specific, exhibit a hydroxylated phenolic compound containing an aromatic arene ring. These kinds radicals originated from phenolic compounds are reported to be less reactive and possess lower electron reduction potential compared to oxygen radicals [26]. Owing to these properties, the phenolic compounds are considered to be excellent radical chemical substances. Thus, phenolic metabolites have the prospect of scavenging reactive oxygen intermediates without raising further oxidative reactions. Interestingly, the presence of such phenolic compounds in jojoba was documented earlier in the literature [27].

Lipoxygenase is an important enzyme that synthesizes leukotrienes, which were reported to play a major role in the elucidation of free-radical diseases. Abdel-Mageed et al. [3] have reported the isolation of antioxidants and lipoexgenase inhibitors in jojoba. The researchers isolated 10 flavonoids and four lignans. They reported that flavonoid aglycosides showed stronger antioxidant and lipoxygenase inhibitory effects than their glycoside counterparts. They demonstrated that the antioxidant and lipoxygenase the inhibiting activity of flavonoids is dramatically reduced by the presence of a sugar moiety in the compound. In a similar study, the essential oil extracted from jojoba displayed an inhibition percentage of 7.81% based on a DPPH radical scavenging assay [28]. Fumonisins are mycotoxins that interfere with ceramide synthase, leading to the inhibition of synthesis of biological molecules and incurring lipid peroxidation in rat hepatocytes [29]. Ethanolic extract from jojoba was found to inhibit oxidative stress induced by fumonisins [30]. In addition to the presence of phenolic compounds, phytosterols, toccopherols and fatty acids, Al-Qizwini et al. [20] associated the antioxidant property of jojoba with the presence of simmondsin and its products: simmondsin-3′-ferulate, 4,5-didemethylsimmondsin and 4-demethylsimmondsin-2′-ferulate. Similarly, the antioxidant property of jojoba oil based on nitric oxide and DPPH scavenging assays has been illustrated [31].

Jojoba extracts were also shown to possess antimicrobial and antifungal activities against several pathogens [32]. Previously, the antimicrobial and antiproliferative activities of jojoba oil obtained from Sudan were reported [33]. However, researchers reported that jojoba oil failed to show any antibacterial activity against MRSA, Bacillus subtilis B29, Pseudomonas aeruginosa 60690 or Salmonella choleraesuis. In contrast to this study, jojoba extracts and latex showed effective antimicrobial activity against some bacterial and fungal species such as Bacillus cereus, Salmonella typhimurium, Clostridium perfringens, Escherichia coli, Aspergillus flavus and Candida albicans [32]. The antimicrobial activity of jojoba oil is not surprising as it has been illustrated to show a striking chemical similarity to sperm whale oil [23].

Previously, the antifungal properties of simmondsin and simmondsin 2′-frulate have been evaluated [34]. The study revealed that simmondsin showed higher inhibition than simmondsin 2′-ferulate against all of the test fungi. Simmondsin was found to inhibit Botrytis fabae the most and was less sensitive in inhibiting Fusarium oxysporum. On the other hand, simmondsin 2′-ferulate has the highest inhibitory activity on Rhizocotonia solani and B. fabae, as compared to its activity on Pythium debarianum and F. oxysporum.

In a similar study, Menghani et al. [35] reported the antimicrobial and antifungal properties of jojoba seed extracts. Their findings assert that the crude extracts have good antimicrobial activity against selected test bacteria and fungi. The importance of COX-2 in terms of carcinogenesis is shown to include: (i) increased production of prostaglandins, (ii) inhibition of apoptosis, (iii) conversion of procarcinogens into carcinogens, (iv) promotion of angiogenesis, (v) increased tumor-cell invasiveness, and (vi) modulation of inflammation and immune function [36]. Therefore, any phytochemical extract that inhibits COX-2 is said to exhibit anticancer activity by inducing apoptosis and suppressing proliferation. In agreement with this assertion, a noncynogenic cyclooxygenase inhibitor was isolated from jojoba, and it was shown to possess anticarcinogenic activity [16].

Jojoba, living in the bright desert sun, is a true heliophyte and tolerates the extreme daily fluctuations of temperature which commonly range through −1 °C during the morning to daily extremes of 46 °C (shade readings). Seedlings are sensitive to light frosts of −1 or −2 °C. Mature shrubs are known to tolerate temperatures as low as −9 °C [49]. Flowers are reported to be destroyed by late frosts although they are hardy in the bud stage [50]. The relatively low winter temperatures synchronized with soil moisture build up appear natural for jojoba reproduction. Optimum growth occurs in the range of 27–36 °C. A daily range of −1 to 50 °C has been recorded in the Mexican desert habitat, but temperatures above 50 °C are believed to suppress growth, although they are not lethal [51]. Jojoba is a dioecious species—i.e., having separate male and female plants (Fig. 1d). Only the females, however, provide the valuable seeds [52]. When raised through seeds, about 50% or more of the seedlings are males [53]. The sex can be recognized only when the plants start bearing after 3–4 years of planting, while for an efficient commercial yield, no more than a 10% male population is required [54]. The success of jojoba growers, and indeed of the entire jojoba industry, depends upon the selection of high-yielding genotypes and their multiplication through vegetative means. Propagating jojoba by direct seeding has genetic heterogeneity, which has raised doubts about the economic feasibility of cultivating jojoba [55]. Vegetative propagation can be achieved by rooting or grafting semi-hardwood cuttings. However, the highest number of propagules is restricted by the size of the plant and time of year [56]. Micropropagation of the best-selected individuals exploits potential genetics of plant cells and offers a capable means of profitable mass production of disease-free elite clones. In vitro-derived cultivated jojoba plants grow more dynamically than both seedlings and rooted cuttings, and the plant size is significantly bigger after a few months of growth. Size and age of cuttings and time of taking the cuttings also affect the rooting percentage in jojoba [10]. Rooting of jojoba stem cuttings is considered the most common and easiest asexual propagation method in jojoba [10]. Soil media used for planting the cuttings or for subsequent transplanting may affect the rooting of cuttings as well as their growth and survival. Khattab et al. [57] investigated the effect of different factors, such as the date of collecting cuttings, plant wounding, and dipping the plant cuttings into chemical treatments, on the propagation success percentage. As a result, it was concluded that using wounded cuttings in summer (end of July) recorded a significantly higher success rate than that of unwounded cuttings. [57]

Jojoba seedlings develop deeply penetrating tap roots, allowing them to access water and nutrients stored deep in the soil, while saplings tend to produce a more fibrous root system unable to sustain them under stressful conditions. Fertilization during rooting was not found to increase the rooting percentage. However, it was found to increase tissue nutrient levels and growth of rooted cuttings [58]. Application of liquid fertilizer containing zinc, potassium, and/or ascorbic acid positively affected the physiological functions and growth criteria of the tested plants compared with a control jojoba plant [59]. Results indicated that jojoba plants seem to have better growth after being sprayed in mid-March with micronutrient fertilizers in both green- and shade-house conditions [60]. A study conducted by Bala et al. [1] on the effect of various soil media on seed germination of jojoba under in vivo conditions showed the highest percentage of seed germination in the desert soil mixed with farmyard manure at a ratio of 2:1, and it recommends prior germination of seeds under nursery conditions to raise potentially healthy plantlets for the successful establishment in the field. Cultivation of the jojoba plant has been threatened by bacterial and fungal diseases. One of the first fungal diseases reported was in Australia [61], and Pleospora herbarum was reported to cause jojoba leaf spots. In Arizona, it was reported that Phytophthora parasitica caused jojoba leaf blight [62]. Ganoderma lucudium infection and colonization has been reported in India [63]. Collar rot caused by Fusarium oxysporum was also reported in Australian jojoba plantations [64], while the first bacterial disease caused by the soil bacterium Burkholderia andropogonis was also found in Australia [65]. Collar and root rot of jojoba caused by Phytophthora nicotianae were previously reported in Argentina [66]. Ash et al. [67] reported black scab of jojoba in Australia caused by the fungus Elsinoë australis. Omar and his co-authors recently reported a new bacterial infection (Candidatus Phytoplasma) of jojoba in Saudi Arabia [68]. In spite of these disease reports, there is no study describing the economic impact of these pathogens on jojoba plantations and how these pathogens affect the oil productivity.

Using ISSR and RAPD markers, it was shown that axillary bud multiplication is a safe method for production of true-to-type plants in jojoba [72]. A reliable gender diagnostic PCR assay [73] and CAPS assay [52] for jojoba were also reported. AFLP markers were also successfully employed in gender determination of jojoba cultivars [74]. Al-Obaidi et al. [75] developed a set of RAPD-PCR-based biomarkers for both in vitro and in vivo jojoba cultivars. Al-Soqeer et al. [76] observed highly significant differences among six jojoba genotypes in oil, protein, total carbohydrate and simmondsin contents. They compared the molecular data obtained from ISSR markers with the chemical traits obtained from the same genotypes. The tree diagram generated using collective ISSR data separated the jojoba genotypes into two main groups. Genotypes found in the same sub-cluster using ISSR primers also had almost similar values for most chemical traits. Moreover, Al-Soqeer and his co-authors [77] found a large genetic variability among seven jojoba genotypes in growth, vegetative, reproductive and seed-yield characteristics. Early diagnosis of sex in Jojoba using a male-specific inter-simple sequence repeat marker was developed [6]. The group managed to amplify a fragment of approximately 1000 bp in male plants. That fragment was completely absent in female plants. Another unique male-specific sequence tagged sites (STS) marker was generated from diverse genotypes of jojoba [78], and the same group also validated a male sex-specific UBC-8071₂₀₀ ISSR marker and its conversion into sequence tagged sites [79]. Two types of gene targeted markers—start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP)—were developed to detect genetic variations among different Simmondsia chinensis genotypes [54].

In a recent study, both molecular analysis and biochemical fingerprinting cumulatively revealed a significant level of variability among 18 accessions of the jojoba plant collected from Rajasthan, India [80]. Ten major fatty acids were found in all the accessions, and of these, oleic acid (OA) was in high concentration. Further OA content of individual accessions was correlated with RAPD analysis data. Genomic DNA sequencing is considered important information which can be used for assessment and improvement of plants [81]. Recently, it has been revealed that proteins involved in metabolism, energy biotic and abiotic stress exhibit differences with high expression level in male compared to the female plants [82]. The potential benefit of molecular assessment in the jojoba plant could enable screening of its varieties of high oil quality and quantity, and examine the factors in which they are hereditarily identical. Jojoba research is still in its infancy with respect to genetic improvement. Future research should focus on jojoba genome sequencing, which is considered a very important step. The jojoba genome will enable future research on the evolution of the study of the oil biosynthesis process and composition, potential genetic improvement and gender selection for this homomorphic plant. Research and development should focus on the oil biosynthesis pathways and be selective for biological markers for high oil yield and earlier-flowering plants. The molecular-based research on the plant growth, seed generation and oil development process of jojoba will be an essential step towards suitable and profitable jojoba cultivation. This information will help the future study of gene expression involved in the oil biosynthesis process. Bearing in mind the plant’s massive potential, a large amount of high-quality planting material is essential for its forthcoming applications. Many research groups have been working on the improvement of jojoba through traditional selection or breeding programs, but the biomass volume is considered low in comparison to other oil crops [83]. Importantly, biomass, oil yield, and early detection of plant gender can be improved by applying molecular biology techniques that will accelerate this process. Development platforms of jojoba cultivation by modern methods of agro-biotechnology are of concern worldwide, not only for oil production but for combatting desertification. Therefore, there is an urgent need to create high-quality genotypes for successful application of jojoba cultivation. High throughput ‘omics’ research such as transcriptomics and metabolomics research platforms will also be favourable approaches to study oil production stages and for more efficient early gender-based differentiation research.