Fig. 4

Spike S1 decreases gap junction channel activity. (A) Representative fluorescence micrographs of SL/DT with DAPI by HeLa-Cx43^GFP/ACE2^mCherry under control conditions (top pannel) or after treatment with 50 pM spike S1 for 24 h (bottom pannel). (B) Averaged data normalized to control (dashed line) of SL/DT with DAPI by HeLa-Cx43^GFP/ACE2^mCherry treated with 50 pM spike S1 for 24 h. *p < 0.05, the effect of spike S1 compared to control (two-tailed Student’s unpaired t test). Averaged data were obtained from three independent experiments with four repeats each. Scale bar = 100 μm