Skip to main content
  • Research article
  • Open access
  • Published:

Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice



Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear.


Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS.


Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.


Cytoplasmic male sterility (CMS) was maternally inherited in plants and in most cases originated from mitochondrial DNA rearrangements which resulted in plants inability to produce functional pollen [1,2,3,4,5]. The CMS trait was observed in more than 200 flowering plant species [6, 7]. CMS produced hybrids using “three-line” system can show heterosis, which exhibits the improved performance of hybrid progeny in comparison with the parental lines [8]. For example, the yield had increased up to 20% in hybrids of rice by using CMS lines [9, 10].

Generally CMS was associated with mitochondria, which was an essential organelle for cellular energy production [10, 11]. Since pollen development required a large amount of energy supply, disturbances or disorder in mitochondrial functions could have important effects on male fertility [5, 12]. The mitochondrial respiratory chain consisted of four complexes: NADH dehydrogenase complex (Complex I), succinate dehydrogenase complex (Complex II), cytochrome c reductase complex (Complex III), and cytochrome c oxidase complex (Complex IV). The F0F1-ATP synthase was Complex V. In mitochondria, energy was generated by the production of proton gradient via electron transport, which catalyzed electron transfer from NADH or FADH2 to molecular oxygen and lead to ATP generation [13]. Any disturbance of this electron transport process could impair the energy production [10].

Mitochondrial complex I was an important pathway for the oxidation of NADH in bacteria, higher plants and animals, and had to do with plant CMS. In plants, CMS phenotype has been described involving nad9 and nad7. For instance, the rice CMS line RT98A derived from Oryza rufipogon, the orf113 displayed completely identical sequences to nad9 in the region − 151 bp to + 11 bp, whereas the rest consisted of unknown sequences [14]. In Nicotiana sylvestris CMS I and CMS II, deletion of exons III and IV of nad7 in CMS I and the complete disappearance of nad7 in CMS II resulted in CMS [15].

In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between M2B and M2BS [16]. In the present study, in order to further understand the relationship between these genes and CMS characteristics in M2BS, the anther cytological observation were performed, the mitochondrial complex I activity and respiration rate were determined, and the RNA editing and the relative expression of these genes were also analyzed between the two lines.

Materials and methods

Plant materials

M2BS was a transgenic CMS line induced by the partial-length HcPDIL5-2a transformation in rice [16] using M2B as a transgenic receptor material. M2B was an indica rice variety and was the maintainer line of M2BS. After successively backcrossing, all the plants were planted in the field with normal management.

Optical microscopic observation

Anthers morphologic observation was performed by camera (Cannon, Japan). Anthers were harvested from upon, middle and bottom spike of three plants and were stained with 1% I2-KI to observe pollen fertility at anthesis.

Scanning electron microscope (SEM)

Anthers were collected at anthesis and fixed for 24 h in Carnoy's Fluid, then dehydrated by a standard series of ethanol washes: 70%, 80%, 90%, and 100% each for 5 min, immediately dried for several minutes, coated with gold by a gold sputter, and was analyzed using Phenom LE electron microscope.

Transmission electron microscope (TEM)

The anthers on the top portion of spikes were used and placed in a bottle contained 2.5% glutaraldehyde buffer solution at anthesis. Air was pumped out of centrifuge tubes in order to soak anthers fully in buffer solution. Anthers were fixed, dehydrated, embedded and examined according to the reference [17].

Cytochemical detection of H2O2

H2O2 was visualized at the subcellular level using CeCl3 for localization [18]. Electron-dense CeCl3 deposits are formed in the presence of H2O2 and are visible by transmission electron microscopy. Anthers were excised from CMS line and maintainer line at anthesis and incubated in freshly prepared 5 mM CeCl3 in 50 mM MOPS at pH 7.2 for 1 h. The experimental method was referred to [19].

Determination of mitochondrial complex I activity and respiration rate

Spike samples from booting stage and anthesis were collected and stored at − 80 ℃ for determination of biochemical attributes. The activity of mitochondrial complex I was determined using mitochondrial complex I Kit (COMIN, Suzhou, China). The experimental operation was completed according to the kit instructions of manufacturer. Calculated by the fresh weight of sample, each gram organization consumes 1 nmol NADH per minute, defined as an enzyme activity unit. The formula for measuring using 96 orifice plates was as follows: Mitochondrial complex I activity (U/g fresh weight) = [ΔA × Vt ÷ (ε × d) × 109] ÷ (W × Vs ÷ Vts) ÷ T = 730 × ΔA ÷ W. Vt: total volume of reaction system, 2.25 × 10–4 L; ε: NADH molar extinction coefficient, 6.22 × 103 L/mol/cm; d: diameter of 96 orifice plates, 0.5 cm; Vs: sample volume added, 0.01 mL; Vts: the added volume of extract solution, 0.202 mL; T: reaction time, 2 min; W: sample weight, g. The respiration rate of anthers was determined by the method of Balkos et al. [20].

RT-PCR amplifications

Total RNA samples were isolated from young panicles with CTAB as described by Liu and He [21]. First-strand complementary DNAs (cDNAs) were synthesized from 1 μg RNA using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa Bio-medicals, Dalian, China). Elimination of DNA was checked by PCR for N4 primers, which amplified a 463 bp fragment for cDNA and a 1717 bp fragment (including a 1254 bp intron) for gDNA (Additional file 1: Fig. S1). The PCR amplifications were carried out using a thermal cycler (Bio-Rad, Hercules, CA, USA) in a total volume of 25 μL reaction mixture, which included 12.5 μL 2 × Mix (containing high-fidelity DNA polymerase) (Takara, Dalian, China), 6.5 μL ddH2O, 2 μL cDNA, 2 μL each primer (10 μmol/L each). The primer sequences were listed in Additional file 2: Table S1. The PCR conditions were as follows: an initial denaturation step at 94 °C for 3 min, followed by 34 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 1 min, followed by a final extension at 72 °C for 5 min.

Sequencing and calculation of editing frequency

The PCR products were eluted, purified, cloned and last sequenced by Beijing Genomics Institute (BGI). Editing efficiency was determined by totally sequencing at least 12 cDNA clones from two separate experiments. Editing frequency was calculated by the ratio of clones containing this editing to the total clones sequenced [22]. The nucleotide sequences were analyzed through DNA Man soft program and submitted to ORF finder and NCBI online service. PCR primers used in this study are listed in Additional file 2: Table S1.

Quantitative reverse transcription PCR (qRT-PCR) analysis

qRT-PCR was performed with qTOWER2.2 sequence detection system (Jena, Germany) using SYBR® Premix Ex Taq™ (Takara, Dalian, China). A housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of rice served as the internal reference. The primer pairs RT-ND5-F/R, RT-nad4-F/R, RT-nad7-F/R, RT-GAPDH-F/R were used for qRT-PCR analysis at anthesis (Additional file 2: Table S1). All reactions were conducted in triplicate for each sample. The relative expression spikes of each transcript were estimated by the 2Ct method [23].


Morphological observation of anther

The maintainer line (M2B) anther was yellow (Fig. 1a), while the anther of the CMS line (M2BS) were light yellow (Fig. 1b). The pollen grains of M2B could be stained by KI-I2 and were dark grey (Fig. 1e), whereas the sterile pollen grains were invalid in KI-I2 (Fig. 1f). SEM results showed sturdy anther and spherical pollen grain in M2B (Fig. 1c, g). However, M2BS presented fissured anther and irregularly typical abortion pollen grain (Fig. 1d, h).

Fig. 1
figure 1

Morphological characteristics of anthers. a, c represented the anther of M2B; b, d represented the anther of M2BS; e, g represented the pollen of M2B; f, h represented the pollen of M2BS. 10 × 

Transmission electron microscopy analysis

Studies reported that mitochondrial ultrastructure was involved in plant CMS [24]. In this study, TEM was performed to study the mitochondrial ultrastructure changes in two lines. The results showed intact nucleus in M2B anther cells, whereas the nucleus of M2BS exhibited degradation and disappearance (Fig. 2a, e). The anther mitochondria of M2B revealed intact mitochondrial structure and clear ridge. In contrast, the mitochondria degraded to some extent, and the cristae of mitochondria were obscure and disintegrated partly in M2BS (Fig. 2b, f). The mature pollen grains of M2B showed circular shape, while M2BS presented non-circular pollen grains. In M2B pollen grains, we observed the cellular inclusions, while nothing was observed in that of M2BS (Fig. 2c, g). Moreover, the intact chloroplast structure, dense and clear thylakoids were observed in anther cells of M2B (Fig. 2d). However, anther chloroplast structure of M2BS was fuzzy and degraded partly, and the thylakoids dissolved (Fig. 2h).

Fig. 2
figure 2

Transmission electron micrographs of anther mitochondria and chloroplast ultrastructure at anthesis. a-d represented maintainer line M2B; eh represented CMS line M2BS; a, e Nucleus observation; b, f Mitochondria observation; c, g Mature pollen grains observation. d, h represented anther chloroplast ultrastructure of M2B and M2BS, respectively. N: Nucleus; mt: Mitochondria; Ch: Chloroplast; P: Pollen grains; cw: cell wall. G: Granal thylakoids

Subcellular localization of H2O2 in anther

There were a lot of evidences that plant CMS was accompanied by the production of large amounts of reactive oxygen species (ROS) [12], and it could be observed that the combination of H2O2 and CeCl3 could form the electron-dense precipitates at transmission electron microscope (TEM). In the present study, the sites of electron-dense precipitates of CMS line and its maintainer line were observed in anther cell at anthesis. The results showed that a large number of electron dense precipitates appeared in intercellular space of anther cells of M2BS at anthesis (Fig. 3b), whereas no obvious electron dense precipitates were observed in those of M2B (Fig. 3a).

Fig. 3
figure 3

The sites of electron-dense precipitates of anther at anthesis. a, b represented M2B and M2BS, respectively. edp: electron-dense precipitates; cw: cell wall

Changes of respiration rate and mitochondrial complex I activity

Mitochondria is the center for plant energy biology and plays a vital role in the respiration and metabolism of plants. In the present study, the spike respiration rate of two lines were determined both at booting stage and anthesis. The results indicated that the respiration rate of M2B presented no obvious changes at different stages of anther development. However, significant decrease was detected in M2BS at anthesis. Compared with M2B, the respiration rate at booting stage and flowering stage decreased by 14.9% and 47.8% in M2BS, respectively (Fig. 4a).

Fig. 4
figure 4

Respiration rate and mitochondrial complex I activity during different pollen development stages

In order to understand the complex I probably associated with CMS characteristics, the activity of complex I was investigated to determine how a partially mitochondrial and partially nuclear encoded enzyme reacted to this cytoplasmically determined phenomenon of CMS. The complex I showed a significantly reduced activity in M2BS, compared to M2B during anther development process. The complex I activity of M2BS dramatically decreased by 22.8% and 51.9%, respectively at booting stage and flowering stage (Fig. 4b).

RNA editing and expression of nad4, nad5, and nad7

RNA editing result showed only nad7 presented partially edited among these three genes. The CDS region of nad7 was verified using primers of nad7cds-F and nad7cds-R, a specific product approximately 1185 bp was amplified from cDNA of M2B and M2BS, respectively (Additional file 3: Fig. S2). The comparisons of cDNA and genomic sequence data revealed only one distinct editing site in the nad7 CDS region of M2B and M2BS (Fig. 5). This common editing site located at 534th nucleotides with the initiation codon of nad7. The editing efficiency was shown in Table 1, and the results showed that the editing frequencies were higher in M2BS than in M2B. The nucleotide substitutions were C-U transitions for this editing site in nad7 transcripts, where was a silence modification.

Fig. 5
figure 5

Editing site of nad7

Table 1 Editing frequencies of nad7 transcripts in M2B and M2BS

In order to further study the transcriptional expression of nad4, nad5, and nad7 at anthesis, we performed qPCR for M2B and M2BS. The results showed that The expression of nad5 and nad7 revealed significant differences between two lines (Fig. 6).

Fig. 6
figure 6

The relative expression of nad4, nad5, and nad7


Mitochondria is an essential organelle in cell not only because it supplies over 90% energy for cells but also because its dysfunction is associated with disease [25]. As the main organelle of energy metabolism, the structure and quantity of mitochondria might be related to male sterility [24]. Changes in mitochondrial structure lead to abnormal electron transfer chains or subunit complexes of ATP enzymes involved in energy metabolism in mitochondria, resulting in the absence of sufficient energy supply for microspore development and pollen abortion [26]. In the present study, the disappearance of cell nucleus and the abnormal mitochondrial structure were observed in anthers of CMS line (Fig. 2). It was in accord with previous studies [27,28,29]. These findings may support the opinion that abnormal mitochondrial structure may be the cellular morphology characteristics of male sterility. In addition, a large number of electron dense precipitates appeared in intercellular space of anther cells of M2BS (Fig. 3b). It was indicated that the H2O2 burst and the production of these ROS at anthesis would also have influences on pollen development and growth. These findings were consistent with the results observed in Brassica napus [30].

Mitochondria was the site of aerobic respiration. Respiration rate was an important physiological attribute for identifying plants respiration intensity and energy metabolism [31]. Previous studies showed lower respiration rate in male sterile anthers compared with fertile ones, indicating some defects in some steps of respiration in male sterile anthers [32,33,34,35]. In this study, the respiration rate of the CMS line was lower than that of the maintainer line (Fig. 4a), which was in agreement with the reports mentioned above.

Mitochondrial complex I was one of the largest macromolecular complexes [36, 37] which played an important role in the cellular energy production. Complex I, a functional enzyme, generated reactive oxygen species (ROS), which could be detrimental, but was also of importance for cell signaling [38]. Defects in this enzyme lead to a severe disturbance of energy metabolism and often lead to severe inherited metabolic disorders [39]. Ducos et al. [40] found that the NAD9 subunit had a C-terminal extension while COX2 subunit had a truncated C-terminus in two mutations of CMS wild beet. Further, they reported that the complex I activity was unchanged in leaves, but the complex IV activity was reduced by 50%. In the present investigation, the different result was obtained and the lower complex I activity was detected in M2BS (Fig. 4b).

CMS and RNA editing were two important phenomena involving in plant mitochondria. It was generally agreed that CMS was caused by altered gene expression due to defective or inadequate RNA editing [22, 41,42,43]. Kim et al. found defects at seven specific editing sites in five mitochondrial genes (cox2, cox3, nad2, nad4 and ccmc) of an ogr1 rice mutant, which were in connection with pollen grain abnormalities [44]. Recently, editing was absent in nad4 at position 1033 of mutant plants, which carried a cytidine residue at this position, where wild type plants instead carried a uridine residue [45]. Compared to Weibenberger et al. [45], in the present study, the nucleotide substitutions were all C-U transitions for the editing site in nad7 transcripts, where was a silence modification site (Fig. 5) and the expression of nad5 and nad7 revealed significant differences between two lines (Fig. 6). Therefore, it was inferred that the mutation, RNA editing, and expression of the complex I genes may be associated with the CMS. In our previous study, the results showed chloroplast differences in leaves of two lines, such as photosynthetic parameters, chloroplasts ultrastructure, soluble sugar and starch content, sugar and starch metabolism genes expression, and photosynthetic related genes [46]. As is known to all, chloroplast is an important site for the photosynthesis and mitochondria is the place of respiration. Photosynthesis uses light energy and water to convert atmospheric carbon into carbon-rich compounds such as carbohydrates. Respiration oxidizes these compounds, releasing useable energy and forming carbon intermediates needed for biosynthesis. On a whole plant basis, up to 70% of the fixed during photosynthesis can be released back into the atmosphere by mitochondrial respiration [47]. Therefore, a further understanding of both photosynthesis (chloroplast) and respiration (mitochondria), and the interplay between them, is necessary.


Morphological and cytological observations showed significant differences between M2BS and M2B. The anther respiration rate and complex I activity of M2BS were significantly lower than those of M2B during pollen development. The expression of nad5 and nad7 revealed significant expression differences between two lines. Overall, the mitochondrial structural degradation and complex I deficiency might be associated with the transgenic CMS of rice.

Availability of data and materials

Not applicable.



Cytoplasmic male sterility


Transmission electron microscopic


Scan electron microscopic


Quantitative reverse transcribed PCR


  1. Schnable PS, Wise RP. The molecular basis of cytoplasmic male sterility and fertility restoration. Trends Plant Sci. 1998;3(5):175–80.

    Article  Google Scholar 

  2. Hanson MR, Bentolila S. Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell. 2004;16:154–69.

    Article  Google Scholar 

  3. Horn R. Cytoplasmic male sterility and fertility restoration in higher plants. Prog Bot. 2006;67:31–52.

    Article  CAS  Google Scholar 

  4. Chase CD. Cytoplasmic male sterility: a window to the world of plant mitochondrial-nuclear interactions. Trends Genet. 2007;23(2):81–90.

    Article  CAS  PubMed  Google Scholar 

  5. Chen L, Liu YG. Male sterility and fertility restoration in crops. Annu Rev Plant Biol. 2014;65(5):5.1-5.28.

    Google Scholar 

  6. Hanson MR. Plant mitochongdrial mutations and sterility. Annu Rev Genet. 1991;25:461–86.

    Article  CAS  PubMed  Google Scholar 

  7. Sun RF, Fang ZY, Zhang SJ, Li F, Niu XK, Wu FY. Biochemicalanalysis of cold-tolerant Ogura CMS chinese cabbage lines. Acta Hortic Sin. 2000;27:187–92.

    Google Scholar 

  8. Baranwal VK, Mikkilineni V, Zehr UB, Tyagi AK, Kapoor S. Heterosis: emerging ideas about hybrid vigour. J Exp Bot. 2012;18:6309–14.

    Article  CAS  Google Scholar 

  9. Virmani SS. Advances in hybrid rice research and development in the tropics. In: Virmani SS, Mao CX, Hardy B, eds., Proc. 4th Int. Symp. on Hybrid Rice. IRRI, Manila, 2003; pp:7–20.

  10. Horn R, Gupta KJ, Colombo N. Mitochondrion role in molecular basis of cytoplasmic male sterility. Mitochondrion. 2014;19:198–205.

    Article  CAS  PubMed  Google Scholar 

  11. Fernie AR, Carrari F, Sweetlove LJ. Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport. Curr Opin Plant Biol. 2004;7:254–61.

    Article  CAS  PubMed  Google Scholar 

  12. Luo DP, Xu H, Liu Z, Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R, Li YG. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet. 2013;45:573–7.

    Article  CAS  PubMed  Google Scholar 

  13. Millar AH, Whelan J, Soole KL, Day DA. Organization and regulation of mitochondrial respiration in plant. Annu Rev Plant Biol. 2011;62:79–104.

    Article  CAS  PubMed  Google Scholar 

  14. Igarashi K, Kazama T, Motomura K, Toriyama K. Whole genomic sequencing of RT98 mitochondria derived from Oryza rufipogon and northern blot analysis to uncover a cytoplasmic male sterility-associated gene. Plant Cell Physiol. 2013;54:237–43.

    Article  CAS  PubMed  Google Scholar 

  15. Gutierres S, Sabar M, Lelandais C, Chetrit P, Diolez P, Degand H, Boutry M, Vedel F, de Kouchkovsky Y, de Paepe R. Lack of mitochondrial and nuclear-encoded subunits of complex I and alterations of the respiratory chain in Nicotiana sylvestris mitochondrial deletions mutants. Proc Natl Acad Sci. 1997;94:3436–41.

    Article  CAS  PubMed  Google Scholar 

  16. Tang DF, Chen P, Jin G, Li M, Wang CC, Bin ZL, Qian JH, Wang ZZ, Zhou RY. Identification of a novel cytoplasmic male sterile line M2BS induced by partial-length HcPDIL5–2a transformation in rice (Oryza sativa L.). J Plant Biol. 2017;60:146–53.

    Article  CAS  Google Scholar 

  17. Shao RX, Xin LF, Zheng HF, Li LL, Ran WL, Mao J, Yang QH. Changes in chloroplast ultrastructure in leaves of drought-stressed maize inbred lines. Photosynthetica. 2016;54:74–80.

    Article  CAS  Google Scholar 

  18. Bestwick CS, Brown IR, Bennett MH, Mansfield JW. Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola. Plant Cell. 1997;9:209–21.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Zhang AY, Jiang MY, Zhang JH, Tan MP, Hu XL. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiol. 2006;141:475–87.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Balkos KD, Britto DT, Kronzucker HJ. Optimization of ammonium acquisition and metabolismby potassium in rice (Oryza satica L. cv. IR-72). Plant Cell Environ. 2010;33:23–34.

    CAS  PubMed  Google Scholar 

  21. Liu Y, He X. Extraction of total RNA from cotton (Gossypium hirsutum L.) tissues with CTAB-PVP method. J China Agric Univ. 2006;1:53–6.

    CAS  Google Scholar 

  22. Wang J, Cao MJ, Pan GT, Lu YL, Rong TZ. RNA editing of mitochondrial functional genes atp6 and cox2 in maize (Zea mays L.). Mitochondrion. 2009;9:364–9.

    Article  CAS  PubMed  Google Scholar 

  23. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−CT) method. Methods. 2001;25:402–8.

    Article  CAS  Google Scholar 

  24. Jiang PD.Studie on the Mechanism of Cotton Cytoplasmic Male Sterility. Dissertation. Zhejiang University. 2007.

  25. Hu J, Huang WC, Huang Q, Qin XJ, Yu CC, Wang LL, Li SQ, Zhu RS, Zhu YG. Mitochondria and cytoplasmic male sterility in plants. Mitochondrion. 2014;19:282–8.

    Article  CAS  PubMed  Google Scholar 

  26. Shi GJ, Hou XL, Yuan JY. Pigment contents and ultrastructure of leaf on cytoplasmic male sterile lines and their maintainer of non-heading Chinese cabbage. J Nanjing Agric Univ. 2004;27(4):31–5.

    Google Scholar 

  27. Warmke HE, Lee SLJ. Pollen abortion in T cyto-plasmic male sterility corn: a suggested machanism. Science. 1978;200:561–3.

    Article  CAS  PubMed  Google Scholar 

  28. Huang JL, Yang P, Li BL, An ZW, Sun DZ. The microstructural and ultrastructural study on microsporogenesis of cytoplasmic male-sterile cotton line jin A. Cotton Sci. 2001;13(5):259–63.

    Google Scholar 

  29. Wang XL, Zhang ZW, Hua JS, Wang XD. Cotton cytoplasmic male-sterility related with the abnormal mitochondrion and vacuole. Bull Sci Technol. 2007;23(2):187–90.

    Google Scholar 

  30. Du K. Cytology and molecular analysis on sterile mechanism of SaNa-1A CMS in Brassica napus. Dissertation. YangZhou University. 2016.

  31. Zhou ZH. Studies on cytological features, biochemical basis and molecular marker of thero-sensitive male sterility in cotton. Dissertation. Hunan Agricultural University. 2006.

  32. Zhang MY, Duan J, Liang CY. The Relationship between respiration and activated oxygen metabolism in cytoplasmic male sterile rice. J Trop Subtrop Botany. 1999;4:318–22.

    Google Scholar 

  33. Zhang MY, Liang CY, Huang YW, Liu HX. Comparison of respiratory pathways of cms and its maintainer rice (Oryza sativa L.). Acta Phytophysiologica Sinica. 1998;1:55–8.

    Google Scholar 

  34. Zhou PJ, Lin XY, Zhang DY, Zhu YG. Energy metabolism and molecular mechanism of the cytoplasmic male sterility in plants. Plant Physiol Commun. 1999;6:491–500.

    Google Scholar 

  35. Xia T, Liu JL. Study on the relation between cytoplasmic male sterile in maize and cn-resistant respiration in tissues. Scientia Agricultura Sinica. 1988;5:39–43.

    Google Scholar 

  36. Brandt U. Energy converting NADH:quinone oxidoreductase (complex I). Annu Rev Biochem. 2006;75:69–92.

    Article  CAS  PubMed  Google Scholar 

  37. Hirst J. Mitochondrial complex I. Annu Rev Biochem. 2013;82:551–75.

    Article  CAS  PubMed  Google Scholar 

  38. Drose S, Brandt U. Molecular mechanisms of superoxide production by the mitochondrial respiratory chain. Adv Exp Med Biol. 2012;748:145–69.

    Article  PubMed  CAS  Google Scholar 

  39. Morris AA, Leonard JV, Brown GK, Bidouki SK, Bindoff LA, Woodward CE, Harding AE, Lake BD, Harding BN, Farrell MA, Bell JE, Mirakhur M, Turnbull DM. Deficiency of respiratory chain complex I is a common cause of Leigh disease. Ann Neurol. 1996;40:25–30.

    Article  CAS  PubMed  Google Scholar 

  40. Ducos E, Touzet P, Boutry M. The male sterile G cytoplasm of wild beet displays modified mitochondrial respiratory complexes. Plant J. 2001;2:171–80.

    Article  Google Scholar 

  41. Gray MW, Hanic-joyce PJ, Covello PS. Transcription, processing and editing in plant mitochondria. Annu Rev Plant Physiol Plant Mol Biol. 1992;43:145–75.

    Article  CAS  Google Scholar 

  42. Bonen L, Brown GG. Genetic plasticity and its consequences: perspectives on gene organization and expression in plant mitochondria. Can J Bot. 1993;71:645–60.

    Article  CAS  Google Scholar 

  43. Hu JH, Yi R, Zhang HY, Ding Y. Nucleo-cytoplasmic interactions affect RNA editing of cox2, atp6 and atp9 in alloplasmic male-sterile rice (Oryza sativa L.) lines. Mitochondrion. 2013;13:87–95.

    Article  CAS  PubMed  Google Scholar 

  44. Kim SR, Yang JI, Moon S, Ryu CH, An K, Kim KM, Yim J, An G. Rice OGR1 encodes a pentatricopeptide repeat-DYW protein and is essential for RNA editing in mitochondria. Plant J. 2009;59:738–49.

    Article  CAS  PubMed  Google Scholar 

  45. Weibenberger S, Soll J, Carrie C. The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1. Plant Mol Biol. 2017;93:355–68.

    Article  CAS  Google Scholar 

  46. Tang DF, Wei F, Kashif MH, Khan A, Li ZQ, Shi QQ, Jia RX, Xie HY, Zhang L, Li B, Chen P, Zhou YR. Analysis of chloroplast differences in leaves of rice isonuclear alloplasmic lines. Protoplasma. 2018;3:863–71.

    Article  CAS  Google Scholar 

  47. Van Der Werf A, Poorter H, Lambers H. Respiration asdependent on a species, inherent growth rate and on thenitrogen supply to the plant. In: Roy J, Gamier E, editors. A whole plant perspective on carbon-nitrogen interactions. The Hague.: SPB Academic Publishing bv; 1994. p. 83–103.

    Google Scholar 

Download references


All the authors are thankful for Natural Science Foundation of Guangxi (2018JJB130096; 2018GXNSFBA294016), Guangxi Innovation-Driven Development Project (GuiKe AA18242040), the China Agriculture Research System (CARS-21), Scientific Research Funding Project of Guangxi Botanical Garden of Medicinal Plants (GuiYaoJi202011) and “Guangxi Bagui Scholars” and Research Innovation Team Project (GuiYaoChuang2019005).

Author information

Authors and Affiliations



TD and WF performed the experiments and wrote the manuscript; AK revised the manuscript; ZR and FM prepared and collected the materials. All the authors agreed on the contents of this paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Danfeng Tang.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1.

Amplification of gDNA and cDNA with prime N4-intron.

Additional file 2.

Primers used in the present study.

Additional file 3.

PCR amplification bands of nad7 CDS region in M2B and M2BS.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tang, D., Wei, F., Khan, A. et al. Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice. Biol Res 54, 6 (2021).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: