Skip to main content

Table 1 Kinetic constants for FNR in PBSs and in the soluble extract of G. ch

From: Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin

Constants

FNRPBS

FNRSOL

K m (μM)

12.5 ± 1.8

16.3 ± 0.3

kcat (s−1)a

86

56.1

Catalytic efficiency (μM s−1)

6.9

3.4

  1. The same procedure was used to determine the kinetic constants for the semi purified enzyme from the soluble extract and for the enzyme that co-purified with phycobilisomes
  2. aProtein concentration was determined by densitometry in SDS polyacrylamide gels using bovine serum albumin (BSA) (Sigma-Aldrich) as standard
Back to article page

Biological Research

ISSN: 0717-6287

Contact us