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Table 1 Kinetic constants for FNR in PBSs and in the soluble extract of G. ch

From: Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin

Constants FNRPBS FNRSOL
K m (μM) 12.5 ± 1.8 16.3 ± 0.3
kcat (s−1)a 86 56.1
Catalytic efficiency (μM s−1) 6.9 3.4
  1. The same procedure was used to determine the kinetic constants for the semi purified enzyme from the soluble extract and for the enzyme that co-purified with phycobilisomes
  2. aProtein concentration was determined by densitometry in SDS polyacrylamide gels using bovine serum albumin (BSA) (Sigma-Aldrich) as standard