Sortin2 does not alter endocytic trafficking in Sortin2 resistant mutants. S. cerevisiae parental line (WT) and Sortin2 resistant mutants were grown on YPD supplemented with 1% DMSO (control) or 20 μM Sortin2. Cells were incubated with 24 μM FM4-64 for 30 min at 4°C. Then turned to 28°C to be imaged after 25 min by confocal microscopy. Images of 25 min incubation are shown. Two images are representative of 20 cells. The experiment was done more than three times. Scale bar represents 5 μm.