Figure 3

Enhancing of endocytic trafficking depends on Sortin2 structural features. S. cerevisiae parental line was grown on YPD 1% DMSO (control) and YPD supplemented with 20 μM of different Sortin2-structural analogs. Afterwards cells were incubated with 24 μM FM4-64 for 30 min at 4°C. Then turned to 28°C to be imaged subsequently by confocal microscopy at different incubation times. Cells with FM4-64 labeled vacuoles were scored. The number of cells with labeled vacuoles relative to the total scored population (N = 30 cells) in different times of FM4-64 incubation is informed with standard deviation.