Figure 5
Effects of Z. heitzii extracts on the integrity of mitochondrial membrane. HL-60 cells were treated with extract and incubated 1 h with 200 nM of Rh-123 and then analyzed by flow cytometry. Data presented are the fluorescence intensity at different concentrations of extract; Control; 20 μg/mL; 50 μg/mL; 100 μg/mL. (5A) MeOH fruit extract; (5B) MeOH bark extract. (5C) graphic representation of mitochondrial damage of HL-60 cells treated with the two extracts of Z. heitzii for 24 h. The fluorescence in the cells is represented as the percentage the mitochondrial damage immediately analyzed using flow cytometry. Data shown are expressed as mean ± SD, Values with different letter are significantly different (p < 0.05).