Figure 4
Effects of Z. heitzii extract on ROS production in HL-60 cells. Cells were treated with extract for 24 h followed by staining with DCHFH2-DA (5 μM), incubated for 30 min and the fluorescence in the cells was immediately analyzed using flow cytometry. Data presented are the fluorescence intensity at different concentrations; Control; 20 μg/mL; 50 μg/mL; 100 μg/L. (4A) MeOH fruits extract; (4B) MeOH barks extract; (4C) is a bold graphic variation of ROS production by HL-60 cells treated with the two extracts of Z. heitzii for 24 h. The fluorescence in the cells (staining with DCHFH2-DA) is represented as the percentage of ROS production considers rewording immediately analyzed using flow cytometry. Data shown are expressed as mean ± SD Values with different letter are significantly different (p < 0.05).