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Figure 4 | Biological Research

Figure 4

From: Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells

Figure 4

Effects of Z. heitzii extract on ROS production in HL-60 cells. Cells were treated with extract for 24 h followed by staining with DCHFH2-DA (5 μM), incubated for 30 min and the fluorescence in the cells was immediately analyzed using flow cytometry. Data presented are the fluorescence intensity at different concentrations; Control; 20 μg/mL; 50 μg/mL; 100 μg/L. (4A) MeOH fruits extract; (4B) MeOH barks extract; (4C) is a bold graphic variation of ROS production by HL-60 cells treated with the two extracts of Z. heitzii for 24 h. The fluorescence in the cells (staining with DCHFH2-DA) is represented as the percentage of ROS production considers rewording immediately analyzed using flow cytometry. Data shown are expressed as mean ± SD Values with different letter are significantly different (p < 0.05).

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