MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway

Table 3 Embryo quality assessment of bovine embryos after IVC with or without miRNA-148b mimic or control mimics

	No. Blastocysts Processed	No. Total nuclei	No. ICM nuclei	No. TE nuclei	Ratio ICM/TE
Control	30	108.8 ± 1.0^b	36.8 ± 1.0^b	66.2 ± 0.7^b	0.5 ± 0.02
CMimic	30	105.3 ± 1.2^b	36.8 ± 0.8^b	68.5 ± 1.1^b	0.5 ± 0.02
miR148b	30	136.4 ± 1.6^a	43.9 ± 1.3^a	92.5 ± 0.9^a	0.5 ± 0.01
miR148b-OV	30	135.3 ± 1.5^a	42.7 ± 0.8^a	92.6 ± 1.2^a	0.5 ± 0.01
miR148b-UT	30	106.5 ± 1.1^b	37.5 ± 1.2^b	69.0 ± 0.9^b	0.5 ± 0.02

Embryos cultured from presumptive zygotes to blastocyst in SOF + 0.3% BSA (Control), or SOF + 0.3% BSA supplemented with 1 µM control mimics (CMimic), or SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b) or embryos cultured from presumptive zygotes to 16- cell stage in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-OV), or embryos cultured from 16-cell stage to blastocyst in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-UT). ICM: inner cell mass; TE: trophectoderm. Data are the mean ± s.e.m. Within columns, different superscript letters indicate significant difference (P ≤ 0.001) between treatments

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