Fig. 4

TGF-β pathway genes mRNA in 16-cell embryos and BD7 cultured with or without miR-148b. (A) Relative mRNA abundance in 16-cell stage embryos cultured in SOF + 0.3% BSA (Control), supplemented with 1 µM control mimics (CMimic), or supplemented with 1 µM of miR-148b (miR148b) or embryos cultured from presumptive zygotes to 16- cell stage in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-OV), or embryos cultured from 16-cell stage to blastocyst in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-UT). (B) Relative mRNA abundance in blastocysts from Control, CMimic, miR148b, miR148b-OV and miR148b-UT experimental groups. Data are the mean ± s.e.m. Different letters indicate significant difference (P ≤ 0.001) between treatments