Fig. 9

BCL2L13 was identified as an important interacting protein of YME1L by proteomics. A KEGG pathway analysis of YME1L-interacting proteins. B GO analysis of YME1L-interacting proteins. C, D Reciprocal Co-IP showing the interaction of YME1L and BCL2L13 in HK2 cells. Arrows indicate specific bands. E, F HK2 cells were incubated with HG for 48 h, and then cell lysates were immunoprecipitated with anti-YME1L, and co-precipitated BCL2L13 were detected by western blotting and the associated quantitative analysis (n = 3). G, H Western blotting and associated quantitative analysis of BCL2L13 in HK2 cells at different time points after HG treatment (n = 4). I, J Western blotting and quantitative analysis of kidney BCL2L13 expression in CON and HFD/STZ mice (n = 6). K-M Western blotting and associated quantitative analysis of LC3II, COX IV expression in HK2 cells transfected with si-BCL2L13 and si-Ctrl on stimulation with D-glucose for 48 h (n = 4). Data are shown as mean ± SD. *p < 0.05, **p < 0.01