Fig. 7

HG-mediated inhibition of mitophagy restores by overexpression of YME1L and exacerbates by silencing of YME1L in HK2 cells. A-C Western blotting and associated quantitative analysis of LC3II, COX IV in HK2 cells at different time points after HG treatment (n = 3). D-I Western blotting and associated quantitative analysis of LC3II, COX IV expression in HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 48 h (n = 4). J-M Representative images and statistical graphs for Immunofluorescence staining of LC3 (Red) and TOM20 (Green) colocalization in HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 48 h (n = 4). Scale bar: 10 µm. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001