Fig. 6

Overexpression of YME1L in the renal tubules improves mitophagy in HFD/STZ-induced DKD. A-C Western blotting and associated quantitative analysis of LC3II and COX IV in each group (n = 4). D, E Representative TEM images and statistical graphs of autophagic structures in kidney tissues in each group (n = 3). Scale bar: 5 µm. F, G Representative images and statistical graphs for immunofluorescence staining of LC3 (Red) and ATP SYNTHASE (Green) colocalization in the renal tubules (n = 5). Scale bar: 20 µm. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. ns: no statistically significant difference