Fig. 4

HG-induced cellular senescence is restrained by YME1L overexpression and accelerated by silencing of YME1L in HK2 cells. A, B Western blotting and associated quantitative analysis of YME1L in HK2 cells at different time points after HG treatment (n = 4). C RT-PCR analysis of YME1L mRNA expression in HK2 cells at different time points after HG treatment (n = 3). D-G Representative SA-β-Gal-staining micrographs of HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 72 h, the percentage of positive cells was quantified (n = 4). Scale bar: 200 µm. H-M Western blotting and associated quantitative analysis of P16, P21 expression in HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 72 h (n = 4). N, O The number of cells in different cell cycles detected by flow cytometry in HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 72 h (n = 4). P-S Bar charts showed the amount of TGF-β1, IL-6 in the cellular supernatant of HK2 cells transfected with YME1L-plasmid, si-YME1L, and their corresponding controls on stimulation with D-glucose for 72 h (n = 3). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001