Fig. 3

Overexpression of YME1L in the renal tubules alleviates the senescent phenotype of RTECs in HFD/STZ-induced DKD. A Volcano plots displayed the differentially expressed genes between Vector and YME1L overexpression HK2 cells in HG conditions. B GSEA revealed negative enrichment of aging up. C Representative SA-β-Gal-staining micrographs indicated senescent tubules with blue color, and D the percentage of positive tubules was quantified (n = 4). Scale bar: 100 µm. E–G Western blotting and associated quantitative analysis of kidney P16, P21 protein expression in each group (n = 4). H RT-PCR analysis of kidney Tgf-β, Il-6, Il-1α, and Tnf-α mRNA expression from HFD/STZ + Ad-EV and HFD/STZ + Ad-Yme1l mice (n = 4). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001