Fig. 11

YME1L promotes the phosphorylation of BCL2L13. A, B Phosphorylation of BCL2L13 was detected by IP with anti-BCL2L13 antibody of HK2 cells treated with HG at different time points (n = 3). C, D Phosphorylation of BCL2L13 was detected by IP with anti-BCL2L13 antibody of kidney tissues from CON and HFD/STZ mice (n = 3). E–G Phosphorylation of BCL2L13 was detected by IP with anti-BCL2L13 antibody of HK2 cells transfected with YME1L plasmid, YME1L si-RNA, and their controls on stimulation with D-glucose for 48 h (n = 3). H-J HK2 transfected with HA-BCL2L13, the protein level of LC3 was detected by IP with anti-HA antibody of HK2 cells transfected with YME1L plasmid, YME1L si-RNA, and their controls on stimulation with D-glucose for 48 h (n = 3). K, L Phosphorylation of BCL2L13 was detected by IP with anti-BCL2L13 antibody of kidney tissues from HFD/STZ + Ad-EV and HFD/STZ + Ad-Yme1l mice (n = 3). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001