Fig. 10

YME1L improves mitophagy and alleviates the senescent phenotype of HK2 cells in the presence of HG via BCL2L13. A-C Western blotting and associated quantitative analysis of LC3II, COX IV expression in HK2 cells transfected with YME1L plasmid, BCL2L13 si-RNA, and their controls on stimulation with D-glucose for 48 h (n = 4). D, E Immunofluorescence demonstrating LC3 (Red) and TOM20 (Green) colocalization in HK2 cells transfected with YME1L-plasmid, BCL2L13 si-RNA, and their controls on stimulation with D-glucose for 48 h (n = 4). Scale bar: 10 µm. F, G Representative SA-β-Gal-staining micrographs of HK2 cells transfected with YME1L plasmid, BCL2L13 si-RNA, and their controls on stimulation with D-glucose for 72 h, and the percentage of positive cells were quantified (n = 4). Scale bar: 200 µm. H-J Western blotting and associated quantitative analysis of P16, P21 expression in HK2 cells transfected with YME1L plasmid, BCL2L13 si-RNA and their controls on stimulation with D-glucose for 72 h (n = 4). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001