Fig. 1

Experimental setup of the study. (A) A schematic diagram to illustrate the cell isolation, injury induction and eMSC intrauterine injection procedures. (B) Timeline of experimental methods. Endometrial injury was implemented by electrocoagulation on left side of the uterus of 6-week-old NOD-SCID mice at Day 0. Intrauterine injection of eMSC was performed on the same day and PBS was injected as untreated control. The mice were sacrificed on post-operative Day 3, 7 and 14 (black stars). (C) Timeline for fertility test, female mice would be mated with 8-week-old male mice on post-operative Day 5. Once copulation plug was observed, the female mice would be sacrificed on gestation Day 8. Abbreviation: eMSC, endometrial mesenchymal stem cells