Fig. 3
From: Embryo development is impaired by sperm mitochondrial-derived ROS
Effect of mitochondrial activity on sperm DNA integrity. (A) Effect of cyanide (5 mM and 10 mM) on sperm viability, sperm motility, MMP and total ROS levels after 72 h of incubation. Cyanide treatments were normalised to the control of each biological replicate (n = 6). Data are represented as mean ± standard error of the mean. Significant differences (P < 0.05) are marked with *. (B) Representative images for the two clusters (P < 0.05), sperm with low and high levels of DNA damage. The lower images represent Comet analysis using the Cometscore v2 software. The cyan line indicates the tail area, the magenta line indicates the head area, and the yellow line indicates the total area. (C) Effect of 5 mM and 10 mM cyanide on sperm DNA damage after 72 h of incubation. The graph shows the impact of cyanide treatments on the subpopulation of sperm with low DNA incidence. Cyanide treatments were normalised to the control of each biological replicate (n = 6). Data are represented as mean ± standard error of the mean. Significant differences (P < 0.05) are marked with *. OCR: oxygen consumption rate. MMP: mitochondrial membrane potential. OTM: olive tail moment. ROS: reactive oxygen species