Fig. 1
From: Embryo development is impaired by sperm mitochondrial-derived ROS
Association of mitochondrial activity with ROS levels and in vitro fertilisation outcomes. (A) Correlation heatmap between mitochondrial activity parameters and in vitro fertility outcomes (n = 33). The colour saturation from red to blue represents Pearson correlation coefficients (R) from 1 to -1, respectively. Only significant correlations (P < 0.05) are numerically indicated. (B) Comparison of OCR (µM O2/h·106 viable sperm) and MMP (ratio) of samples classified as with low (n = 16) or high (n = 17) in vitro fertility potential. Data are represented as mean ± standard error of the mean. Significant differences (P < 0.05 and P < 0.01) are marked with * and **, respectively. (C) Sample clustering (P < 0.05) considering total ROS levels (AU), OCR (µM O2/h·106 viable sperm) and embryo development potential (ratio). Each dot represents a biological replicate (n = 26), and their colouring depends on embryo development potential. (D) Descriptive values of each parameter (OCR (µM O2/h·106 viable sperm), total ROS levels (AU) and developmental potential (ratio)) of the two clusters (P < 0.05). Data are represented as mean ± standard error of the mean. OCR: oxygen consumption rate. MMP: mitochondrial membrane potential. ROS: reactive oxygen species