Fig. 5

SARS-CoV-2 synergizes with an excess of exogenously added α-syn monomers to reduce α-syn multimer:monomer ratio, which is prevented by IFN-β. A Representative western blot, and B quantification of total α-syn, α-syn monomers, α-syn multimers, and C α-syn multimer:monomer ratio in A549-hACE2 cells 48 h post- Mock and SARS-CoV-2 infection in the presence of exogenously added α-syn. Quantification was performed by normalizing to total protein (Loading control). Results show raw normalized values presented as mean ± SEM from n = 3 independent experiments. Multimer:monomer ratio is expressed as absolute value calculated as “multimers/monomers”. Data were analyzed by applying Two-Way ANOVA (for total, multimer, and monomer α-syn quantification) or One Way ANOVA (for multimer:monomer ratio). *p < 0.05, ***p < 0.001, ****p < 0.0001