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Fig. 3 | Biological Research

Fig. 3

From: Melatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression

Fig. 3

MT inhibits caspase-1-dependent pyroptosis in THP-1 macrophages induced by nicotine. (A) RT‒qPCR analysis of the effect of caspase-1 inhibition on pyroptosis-related mRNA expression. (B) WB analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins. (C) Caspase-1 interference segment selection. (D) WB analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins. (E) Effect of MT or VX-765 on NLRP3 and IL-18 immunofluorescence in nicotine-treated macrophages (scale bar: 20 μm). Data are presented as mean ± SD (n = 3 represents three independent experiments per group); *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control cells. #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. nicotine-treated cells

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Biological Research

ISSN: 0717-6287

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