Fig. 8

SYVN1 affects ER stress and EMT by the regulation of SIRT2 in TGF-β1-induced BEAS-2B cells. (A-B), The mRNA and protein expression of SIRT2 in cells transfected with empty vector or SIRT2-oe. Cells were cotransfected with Vector/SYVN1-oe together with Vector/SIRT2-oe, and then treated with TGF-β1. (C), Western blot analysis of SYVN1 and SIRT2 protein expression in cells. (D), Western blot analysis of GRP78 and CHOP protein expression in cells. (E), Western blot analysis of E-cadherin and Vimentin protein expression in cells. (F), Representative immunofluorescence images of cells stained with E-cadherin and Vimentin. E-cadherin (red), Vimentin (red), cell nucleus (DAPI, blue). (G), Schematic diagram for the role of SYVN1 in airway remodeling in asthma. *, P < 0.05