Fig. 2

The main models to mantain the interaction between gastrointestinal cancers PDOs and CAF cells. A Direct PDOs and CAFs co-culture model using the Matrigel droplet method to emulate the ECM. B Model is a PDOs-CAF transwell system, which is used mainly for the evaluation of paracrine communication co-culture interaction between PDOs and CAF. C “Mini-tumor” co-culture, this approach has a previous step allowing the preincubation of CAFs-PDOs in low attachment plates to improve the interaction, before co-culture is achieved using the Matrigel droplet method. The figure was created with Biorender