Table 1 Primers for real-time PCR
Gene | Description | Primer sequence | Tm (℃) |
|---|---|---|---|
Gapdh | m-Gapdh-F | 5ʹ-AGGTCGGTGTGAACGGATTTG-3ʹ | 85.5 |
m-Gapdh-R | 5ʹ-GGGGTCGTTGATGGCAACA-3ʹ | ||
Cav2 | m-Cav2-F | 5ʹ-CTCACCAGCTCAACTCTCATCTC-3ʹ | 88.2 |
m-Cav2-R | 5ʹ-CAACGTCTGTCACACTCTTCCATA-3ʹ | ||
Rab5a | m- Rab5a -F | 5ʹ-CCTTTCTAACCCAAACTGTGTGTCT-3ʹ | 83.2 |
m- Rab5a -R | 5ʹ-CTCGCAAAGGATTCCTCATTTG-3ʹ | ||
Rab7a | m- Rab7a -F | 5ʹ-TTGTGTCTCCTCCTCCGTTGAC-3ʹ | 86.4 |
m- Rab7a -R | 5ʹ-CCGCTCCTATTGTGGCTTTGTA-3ʹ | ||
Clta | m- Clta -F | 5ʹ-CAAGAAGCGGAGTGGAAAGAAA -3ʹ | 86.6 |
m- Clta -R | 5ʹ-CATAACCAATCAGGTCAGCGAA-3ʹ | ||
Ap2a2 | m- Ap2a2-F | 5ʹ-GCTGGCATAATACACACCAAAAC-3ʹ | 87.7 |
m- Ap2a2-R | 5ʹ-GAGAGAGACAAGAGAGACGCACA-3ʹ | ||
Abc1 | m- Abc1-F | 5ʹ-TCGTCTTCCTGTCTGTGTTTGC-3ʹ | 90.6 |
m- Abc1-R | 5ʹ-TGGACCCCGATACCTTGCT-3ʹ | ||
Tom1 | m- Tom1-F | 5ʹ-CTCAGAGACACCATCCAGACAGAAC-3ʹ | 92.3 |
m- Tom1-R | 5ʹ-GGTGCGGTTCAGTTCCTGTAGTAG-3ʹ | ||
Aak1 | m- Aak1-F | 5ʹ-CTACTTCACTTTGCCGTTTGGG-3ʹ | 84.8 |
m- Aak1-R | 5ʹ-CCTTTTGTCAGGGTCTGGTTCC-3ʹ | ||
Snx2 | m- Snx2-F | 5ʹ-CTGTCACTCCCACCACACTCA-3ʹ | 83.6 |
m- Snx2-R | 5ʹ-ATGCCATCACCAACTTTTTCC-3ʹ |