Fig. 5

Functional study of MX1 over-expression. A Bright field and fluorescence images of normal ACs transfected with MX1-OE and Blank lentivirus (scale bar = 100 μm). B Volcano plot of all expressed genes in MX1-OE versus Blank. DEGs were filtered through the Fold change ≥ 1.5 and Qvalue ≤ 0.05. C Quantitative measurement of mRNA expression levels of key genes including MX1 and AP-1 in MX1-OE and Blank group (n = 4, ***p < 0.001, two-tailed Student’s t-test). D KEGG enrichment of up- and down-regulated DEGs. E Gene set enrichment analysis (GSEA) revealed altered pathways related to MX1-OE. F PI staining and quantitative results showed proportion of apoptotic cells in MX1-OE and Blank group (scale bar = 100 μm) (n = 3, **p < 0.01, two-tailed Student’s t-test). G STRING protein network analysis and GO-BP enrichment of DEGs