Fig. 1

Experimental design and preintervention cognitive behavioral testing (A) Gene identification results. The maker brand from top to bottom represent 100, 200, 300, 400, 500, 600, 700 bp. APP = 377 bp, PS1 = 608 bp, GAPDH = 324 bp. Mice numbered 2, 6, 7, 8, 11, 14, 15, 17, 18, and 19 in the figure represent AD positive. B experimental design. (C) OFT schematic. The middle nine squares were defined as the central region. The results of OFT before EA intervention, there were no differences in total movement distance (D) and central area distance (E) or central area time (F) among the groups, indicating that the overall exploration ability and emotional state of the mice were normal. G Schematic representation of the OLT. After 10 min of free exploration, a 5-min test was performed one hour later. A1 and A2 are two objects that look the same. The OLT results before intervention showed decreased memory discrimination in AD mice compared with wild-type mice (H), indicating impaired pattern separation. The motor ability of each mouse was consistent (I). All data are presented as mean ± SD; N = 10 mice; ^***P < 0.001 vs. WT group;