Fig. 5

The potency for neurogenesis of long-term propagated NPCs by FGFR-agonist. (A) The influence of FGFR-agonist on proliferative capacity of the NPCs after 50 passages. NPCs maintained in FGFR-agonist were propagated for 50 passages and treated with either bFGF, FGFR-agonist or Ctrl-oligo for 72 h. The cell proliferation was assessed using the CCK8 assay. Data are depicted as mean ± SD (n = 5), with *p < 0.001 (one-way ANOVA). (B) Long-term propagated NPCs underwent different treatment regimens, including bFGF, FGFR-agonist, or NGF-induced differentiation, over a 10-day period. Following treatment, cells were fixed and analyzed via immunofluorescence. Nestin is represented through green fluorescence, while Tuj-1 is indicated by red fluorescence. Cell nuclei are counterstained with DAPI for contrast. Scale bar = 100 μm. Specific regions of interest (ROI), delineated by white-bordered boxes, are further magnified to provide a detailed view, accompanied by a zoom-in scale bar set at 10 μm