Skip to main content
Fig. 1 | Biological Research

Fig. 1

From: Development of synthetic modulator enabling long-term propagation and neurogenesis of human embryonic stem cell-derived neural progenitor cells

Fig. 1

Design and characterization of FGFR-agonist. (A) The secondary structures of the FGFR-binder and FGFR-agonist determined using Mfold software are presented, indicating the formation of a stem-loop structure. (B) The secondary structure of DNA-based FGFR-agonist is verified using native polyacrylamide gel electrophoresis (PAGE). This panel also includes the determined molecular weights of the single-stranded FGFR-binder, FGFR1-agonist, and a control oligonucleotide (Ctrl oligo). (C) SPR sensorgrams showing the real-time binding kinetics of FGFR-agonist aptamer to immobilized FGFR1 extracellular domain at various concentrations. The sensorgrams are color-coded based on the concentrations of the FGFR-binder (1, 2, 4, 8, 16 and 32 nM) and FGFR-agonist (0.16, 0.31, 0.625, 1.25, 2.5, 5 and 10 nM), respectively. (D) The relative binding performance of FGFR-binder and FGFR-agonist to the NIH3T3 cells was determined by flow cytometry (refer to Fig. S1 for additional details)

Back to article page

Biological Research

ISSN: 0717-6287

Contact us