Fig. 3
Aberrant phenotype of mitochondrial physiology in aged oocytes. A Mitochondrial morphology of human young and aged oocytes under transmission electron microscopy (TEM). Scale bars, 500 nm. B Mitochondrial content (indicated by Mitotracker green) was observed under the confocal microscope. Scale bars, 50 μm. C Cytosolic Ca2+ levels (indicated by Fluo-4) were observed under the confocal microscope. Scale bars, 50 μm. D Mitochondrial membrane potential (MMP, indicated by TMRM) were observed under 3D confocal microscope. Scale bars, 50 μm. E Cytosolic reactive oxygen species (ROS, indicated by DCF) were observed under 3D confocal microscope. Scale bars, 50 μm. The relative abundance of average fluorescence intensity or area was quantified. Data are shown as means ± SEM. Each scatter represents an independent biological individual. Unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01