Fig. 2

USC shows vigorous metabolism on glycolysis, OXPHOS and mitochondrial genome expression activity. A Analysis of glycolytic mRNA expression levels by RT-PCR. B Normalized extracellular acidification rate (ECAR) detected by Seahorse XFe96 analyzer. C Quantification of glycolysis level. D Quantification of glycolytic capacity. E Analysis of tricarboxylic acid cycle (TCA) mRNA expression levels by RT-PCR. F Normalized oxygen consumption rate (OCR) detected by Seahorse XFe96 analyzer. G Quantification of ATP turnover. H Quantification of maximal respiration. I The mRNA expression level of 13 electron transport chain (ETC) genes encoded by mitochondrial genomes was analyzed by RT-PCR. Data are shown as means ± SEM. Each scatter represents an independent biological individual. One-way ANOVA, LSD test. *P < 0.05, **P < 0.01