From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm
Motility (%motile sperm) | Viability (%viable sperm) | ROS (%sperm with high intracellular ROS) | Superoxides (%sperm with high intracellular superoxides) | Agglutination (%agglutinated sperm) | |
---|---|---|---|---|---|
2 min | |||||
Control (0 mM) | 81.69 ± 6.36a | 50.93 ± 12.74 | 8.01 ± 6.28 | 36.95 ± 13.67 | 7.48 ± 2.05a |
0.1 mM | 75.59 ± 8.97a | 53.82 ± 13.40 | 8.30 ± 5.73 | 32.18 ± 14.13 | 10.85 ± 3.35a |
1 mM | 76.12 ± 10.89a | 56.87 ± 12.12 | 7.01 ± 6.31 | 32.42 ± 9.04 | 17.18 ± 5.10a |
5 mM | 67.32 ± 9.64a | 55.09 ± 6.24 | 11.50 ± 7.77 | 40.16 ± 19.29 | 36.70 ± 19.72a |
10 mM | 74.28 ± 5.60a | 44.76 ± 8.49 | 14.25 ± 11.05 | 56.46 ± 13.32 | 59.09 ± 6.82b |
50 mM | 5.02 ± 1.89b | 38.31 ± 7.94 | 12.61 ± 7.60 | 46.77 ± 8.64 | 84.77 ± 5.56b |
10 min | |||||
Control (0 mM) | 83.49 ± 5.29a | 54.70 ± 16.13 | 8.38 ± 3.84 | 46.28 ± 19.39 | 8.81 ± 1.44a |
0.1 mM | 78.16 ± 10.8a | 58.95 ± 16.71 | 11.90 ± 5.12 | 38.04 ± 13.45 | 10.41 ± 4.47a |
1 mM | 73.11 ± 11.92a | 60.16 ± 14.58 | 12.23 ± 5.23 | 49.00 ± 18.74 | 15.11 ± 2.44a |
5 mM | 65.59 ± 2.35b | 47.63 ± 10.77 | 13.56 ± 7.69 | 61.78 ± 5.44 | 34.24 ± 7.84a |
10 mM | 70.74 ± 4.18b | 48.81 ± 16.93 | 12.80 ± 4.90 | 64.50 ± 9.59 | 57.82 ± 4.85b |
50 mM | 6.19 ± 1.84c | 47.90 ± 6.50 | 6.34 ± 3.22 | 49.68 ± 6.26 | 86.15 ± 3.89b |
30 min | |||||
Control (0 mM) | 83.47 ± 7.34a | 60.98 ± 11.23 | 6.46 ± 2.40 | 34.65 ± 15.04 | 11.02 ± 1.43a |
0.1 mM | 75.67 ± 7.75a | 63.44 ± 8.67 | 9.87 ± 2.33 | 32.02 ± 14.21 | 14.80 ± 3.63a |
1 mM | 71.39 ± 13.43a | 58.29 ± 10.18 | 9.85 ± 2.68 | 36.89 ± 15.05 | 20.17 ± 5.94a |
5 mM | 65.06 ± 4.57a | 55.97 ± 9.46 | 12.26 ± 4.55 | 43.39 ± 16.35 | 34.56 ± 5.70b |
10 mM | 66.69 ± 2.99a | 42.57 ± 26.12 | 12.07 ± 3.73 | 45.16 ± 18.95 | 61.23 ± 5.97b |
50 mM | 4.14 ± 1.74b | 38.20 ± 9.45 | 6.04 ± 2.72 | 39.00 ± 10.36 | 81.44 ± 2.36c |
60 min | |||||
Control (0 mM) | 75.29 ± 11.65a | 49.09 ± 17.94 | 4.91 ± 4.41 | 45.43 ± 11.86 | 19.00 ± 12.95a |
0.1 mM | 74.82 ± 11.46a | 42.00 ± 20.10 | 5.00 ± 5.68 | 49.88 ± 9.71 | 19.35 ± 5.11a |
1 mM | 65.67 ± 17.99a | 50.38 ± 11.28 | 5.31 ± 5.31 | 53.14 ± 6.49 | 24.11 ± 6.06a |
5 mM | 67.04 ± 0.97a | 44.39 ± 16.67 | 5.64 ± 5.67 | 63.49 ± 8.07 | 33.15 ± 6.93a |
10 mM | 59.06 ± 5.92a | 31.52 ± 15.86 | 4.68 ± 3.55 | 65.53 ± 24.71 | 56.22 ± 11.57b |
50 mM | 4.32 ± 1.71b | 33.24 ± 5.47 | 3.12 ± 2.97 | 56.88 ± 16.61 | 77.70 ± 7.48c |