Fig. 4From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig spermComet assay Olive Tail Moment (OTM) after incubation of sperm with 10 mM of Mn2+/Ca2+ (A) or 10 mM Mg2+/Ca2+ (B) for different incubation times (0 min, 2 min, 10 min, 30 min, 60 min), in non-permeabilized (−TX) and permeabilized (+TX) samples. (*) indicates significant differences between non-permeabilized (−TX) and permeabilized (+TX) samples (P ≤ 0.05)Back to article page