Fig. 3

Statistics of identified miRNAs and analysis of their base biases in the sequencing libraries. A Number of pre-miRNAs and unique miRNAs in different categories. B Length distribution of all identified unique miRNAs. C UpSet Venn diagram showing the number of shared and specific miRNAs in different samples. AP_NH, NH under mild HT stress; AP_SH, SH under mild HT stress; JP_NH, NH under extreme HT stress; JP_SH, SH under extreme HT stress. D The first base preference of mature miRNAs. The X-axis displays the length classification of the miRNAs, and the Y-axis represents the proportion of mature miRNAs with a certain base type as the first base. E Base preference in different positions of mature miRNAs. The X-axis displays the different base positions of the miRNAs, and the Y-axis denotes the proportion of bases at a certain position of mature miRNAs