Fig. 3

IL-8 and MDA-CM induce VCAM-1 S-nitrosylation depending on NO signaling. Confluent monolayers of EAhy926 cells were incubated with IL-8 (A) or MDA-CM (C) for 5 to 30 min. Cell lysates were obtained and processed by biotin switch to detect S-nitrosylated VCAM-1. Confluent monolayers of EAhy926 cells were incubated with IL-8 (B) or MDA-CM (D) in the presence or absence of l-NMA. Cell lysates were obtained and processed by biotin switch to detect-S-nitrosylated VCAM-1. For A and C, One-Way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05; n = 5. For B and D Two-way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05; n = 5