Fig. 2

IL-8 and MDA-CM increase VCAM-1 endothelial cell surface expression through NO signaling. A EAhy926 monolayers were stimulated with IL-8 for 5 or 15 min and VCAM-1 in the cell surface was determined by cell surface biotinylation. IL-8 significantly increased VCAM-1 at the cell surface. One-Way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05 in comparison to time 0; n = 5. B Pretreatment of cell monolayers with l-NMA inhibited IL-8 stimulated VCAM-1 at the endothelial cell surface. Two-way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05; n = 5. C MDA-CM applied for 5 and 15 min significantly increased VCAM-1 at the cell surface. One-Way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05 in comparison to time 0; n = 5. D Pretreatment of cell monolayers with l-NMA inhibited MDA-CM induced VCAM-1 at the cell surface. Two-way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05; n = 3