Fig. 1 

IL-8 and MDA-CM increase leukocyte adhesion through NO signaling. Cremaster muscle from Rockefeller mice was prepared for intravital microscopy. After stimulation with A IL-8 or B MDA-CM, leukocyte adhesion was recorded up to 30 min. Images show leukocyte adhesion at 5 min of stimulation with IL-8 or MDA-CM, respectively. Treatment with l-NMA before stimulation prevented leukocyte adhesion. Black arrows indicate leukocytes adhered to the endothelial cell surface delimited by dash lines. C, D Bar graphs showing statistical analysis of leukocyte adhesion to the cremaster stimulated with IL-8 and MDA-CM, respectively. Two-way ANOVA and Bonferroni’s Multiple Comparison Test, * p < 0.05 in comparison to time 0. n = 5. E, F Confluent EAhy926 cells were stimulated with IL-8 or MDA-CM for different periods of time and p-eNOS was detected by Western-blot. One-Way ANOVA and Bonferroni’s Multiple Comparison Test. * p < 0.05 in comparison to time 0; n = 5. G Representative image of real-time detection of NO induced by IL-8 in live EAHy926 cells. H Analysis of the increase in real-time detection of NO induced by IL-8 in live EAHy926 cells; paired t test, p < 0.0001; n = 5 independent experiments