Fig. 4

Assessing HTR-8/SVneo cell apoptosis, proliferation and migration after MUC1 knockdown. A, A1: Western blotting data showing the expression of MUC1 in HTR-8/SVneo cells, and A1 shows the quantitative analysis of the control, sh-NC and sh-MUC1 groups. B: Quantitative PCR data showing the mRNA expression of MUC1 in HTR-8/SVneo cells of the control, sh-NC and sh-MUC1 groups. C, C1: Apoptosis of HTR-8/SVneo cells either control cells or transfected with negative control (sh-NC) or sh-MUC1 vectors was determined by flow cytometry using the annexin V-FITC/PI apoptosis assay, and C1 shows the quantitative analysis of the cell apoptosis percentage in the three groups. D, D1: Flow cytometry data showing the analysis of DNA contents in HTR-8/SVneo cells either control cells or transfected with negative control or sh-MUC1 vectors, and D1 shows the quantitative analysis of the proportion of cells in each phase of the cell cycle in the three groups. E, E1: Representative images of HTR-8/SVneo cells, which were either control cells or negative control (sh-NC) or MUC1 knockdown cells (sh-MUC1), subjected to scratch wounding after 0 and 24 h of incubation. E1 shows the quantitative analysis of the cell mobility percentage in the three groups. All statistical data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 1000 μm in E