Fig. 3
From: Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway
Assessment of glucose uptake- and apoptosis-related proteins in HTR-8/SVneo cells after high glucose and FH535 treatment. A: Representative immunofluorescence staining of GLUT4 in HTR-8/SVneo cells of the control, HG and HG + FH535 groups (counterstained with DAPI). A1: Bar chart showing the GLUT4 mean fluorescence intensity in HTR-8/SVneo cells of the control, HG and HG + FH535 groups. B: Quantitative PCR data showing the mRNA expression of GLUT1, GLUT3, GLUT4, GLUT8, INSR, INS1, INS2, AKT, and FOXO1 in HTR-8/SVneo cells of the control, HG and HG + FH535 groups. C-F, C1-F1: Western blotting data showing the expression of GLUT4 (C), INSR (D), Bcl-2 (E), and Caspase3 (F) in HTR-8/SVneo cells, and C1-F1 shows the quantitative analysis of the control, HG and HG + FH535 groups, respectively. All statistical data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. HG: high glucose. Scale bar = 20 μm in A