Fig. 1

Assessing MUC1 expression levels with glucose uptake- and apoptosis-related proteins in control and GDM placentas. A: Determination of the MUC1 level in maternal plasma in the control and GDM groups by ELISA. B, B1: Western blotting data showing the expression of MUC1 in the placentas (B), and B1 shows the quantitative analysis of the control and GDM groups. C: Quantitative PCR data showing the mRNA expression of MUC1 in control and GDM placentas. D: Representative immunofluorescence staining of MUC1 and GLUT4 in the cross-sections of the Control and GDM placentas (counterstained with DAPI). D1-D2: Bar charts show the MUC1 and GLUT4 mean fluorescence intensity in Control and GDM placentas. E-F: Determination of GLUT1 and GLUT4 levels in maternal plasma in the control and GDM groups by ELISA. G: Quantitative PCR data showing the mRNA expression of GLUT1, GLUT3, GLUT4 and GLUT8 in control and GDM placentas. H: Quantitative PCR data showing the mRNA expression of INSR, INS1, INS2, AKT, mTOR, PDK1 and FOXO1 in control and GDM placentas. I-L, I1-L1: Western blotting data showing the expression of GLUT4 (I), INSR (J), Bcl-2 (K) and Caspase3 (L) in the placentas, and I1-L1 shows the quantitative analysis of the Control and GDM groups, respectively. All statistical data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 250 μm in D