Fig. 10

Detection of germ cell apoptosis in control, PTX treatment and PTX + hUC-MSCs treatment mice testis. The mice were treated with PTX or hUC-MSCs, and samples were collected 2 weeks later. A: Detection of positive apoptosis germ cells by TUNEL assay, the arrow indicated the apoptosis germ cells; Expressions of BAX and BCL2 in mice testes of control, PTX and PTX + hUC-MSCs group were detected by Western blotting (B) and immunohistochemistry (C), respectively. Statistical analysis was performed by One-Way ANOVA; p value less than 0.05 was considered significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001; Each bar in (C) represented 20 μm