Fig. 4

EA regulated the expression of miR-219a in the ischemic peripheral HIP and EC of MCAO rats. (A) Statistical analysis of GO enrichment of differentially expressed target genes and of differentially expressed miRNAs. (B) miR-219a-NMDAR1 interaction was detected using a dual-luciferase reporter assay. (C) The changes of relative luciferase activity (***, NC mimics vs miR-219a mimics, P < 0.01). (D) Relative expression of miR-219a in the HIP CA1 region. (E) Representative Western blot. (F) Western blot analysis. Significant differences in the level of NMDAR1 protein. (G) Significant differences in the level of retinal CaMKII protein. (H) Significant differences in the level of retinal p-CaMKII protein. (I) Significant differences in the level of retinal c-fos protein. * EA vs MCAO, MCAO+miR219a mimics VS MCAO, #. EA+miR-219a mimics vs EA, MCAO+miR-219a vs EA. P<0.05.(C. Independent t test., n = 3 rats/ group; D,F-I. One-way ANOVA, followed by Tukey’s post hoc test, n = 3 rats/ group, Data represent the mean ± SD)