Fig. 2From: ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organizationLoss and inhibition of ABCA1 activity impacts migration and invasion abilities of Hs294T cells but not their proliferation. A Representative immunoblots of ABCA1 level of WT and CRISPR/Cas9 generated scrambled and ABCA1 KO cells. Vinculin was analyzed as a loading control (n = 3). B Total cholesterol quantitative measurement of WT, scrambled and ABCA1 KO cells. (n = 9 from three independent experiments, one-way ANOVA with Sidak’s multiple comparisons test). C Esterified cholesterol quantitative measurement of WT, scrambled and ABCA1 KO cells (n = 9 from three independent experiments, one-way ANOVA with Sidak’s multiple comparisons test. D Representative immunoblots of ABCA1 level of Hs294T WT cells treated either with DMSO (vehicle) or 12.5 μM probucol for 2 h. Vinculin was analyzed as a loading control (n = 3). E Total cholesterol quantitative measurement assay of DMSO- or 12.5 μM probucol-treated cells for 2 h (n = 9 from three independent experiments, unpaired Student t-test). F Total cholesterol quantitative measurement of DMSO- or 12.5 μM probucol-treated cells for 16 h (n = 9 from three independent experiments, unpaired Student t-test). G Esterified cholesterol quantitative measurement of DMSO- or 12.5 μM probucol-treated cells for 16 h (n = 9 from three independent experiments, unpaired Student t-test). H, I Quantification of relative proliferation potential after BrdU incorporation assay of scrambled and ABCA1 KO cells (H) or of DMSO- or probucol-treated cells (I) (n = 9 from three independent experiments, unpaired Student t-test). J, K Calculated covered distances of scrambled and ABCA1 KO (J) or DMSO- and probucol-treated cells (K) after 72 h (n = 90 from three independent experiments, unpaired Student t-test). L, M Relative invasiveness of scrambled and ABCA1 KO cells expressed as fold change of scrambled (L) or DMSO- and probucol-treated cells expressed as fold change of DMSO-treated cells (M) after 24 h (n = 9 from three independent experiments, unpaired Student t-test). Data are mean ± SD. ns non-significant, *p ≤ 0.05, ***p ≤ 0.001 and ****p ≤ 0.0001Back to article page