Fig. 6

Calcium transients and mechanical contraction were altered in PLEKHM2 mutated DCM-iPSC-CMs. A Spontaneous beat rate/min (A) and Mechanical contraction (B, C) were measured using MuscleMotion software. Two clones were analyzed from each patient (DCM-iPSCs-CMs clones R4 and R7, n = 25 and DCM-iPSCs-CMs clones O1 and O7, n = 40) or control (DCM-iPSCs-CM clones C1 and C2, n = 77). B Contraction amplitude represents an average of the maximum peak of contraction during spontaneous beatings. C Half width represents the time duration of 50% raise to 50% decline of the contraction-relaxation signal. Intracellular calcium transients (D–F) were measured with the HyperSwitch dual‐excitation and dual‐emission photometry system (IonOptix, MA, USA) during stimulation at 0.25 Hz. D An average of [Ca²⁺]i transients amplitude. E ∆F/F0 represents the % of [Ca²⁺]i change during contraction relaxation cycle. F Half width represents the time duration of 50% raise to 50% decline of the [Ca⁺²]i transients (only one clone was measured; Clone C2, n = 17, Clone R4 n = 17 and Clone O7 n = 8). All statistics were done using One way ANOVA with multiple comparisons and present as average ± SE *p < 0.05, ***p < 0.001