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Fig. 5 | Biological Research

Fig. 5

From: Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction

Fig. 5

Distribution and morphology of DCM-iPSCs-CM. Immunostaining of cardiac troponin T (cTnT) and sarcomeric a-actinin at day 14 after differentiation. Single DCM iPSC-derived cardiomyocytes exhibited a cTNT (green) (A) or punctate sarcomeric a-actinin (B) double stained with F-actin (red) and DAPI (blue) distribution pattern. Enlarged views of the boxed areas show detailed a- actinin and cardiac troponin T staining patterns in the cells. Scale bars: 100 µm. Quantification of the extent of striation along the major axis of DCM-iPSCs-CMs stained with a- actinin in confocal images (n = 13–15) using Image J software (C). Asterisks denote significant difference by 1-way ANOVA, *p < 0.05, **p < 0.01

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Biological Research

ISSN: 0717-6287

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