Fig. 3

Gene expression of DCM-iPSCs-CMs (DCM-iPSCs-CMs clones C1 and C2, DCM-iPSCs-CMs clone O1 and DCM-iPSCs-CMs clone R4): qPCR readout of transcripts of cardiac progenitors NKX2.5 and ISL1 A, B contractile proteins, Troponin T, Troponin C and Troponin I C–E α-MHC and β-MHC, myosin heavy chain F, G and MLC2v and MLC2a, myosin light chains proteins that participate in electrical function H, I; CaV1.2, SERCA2 and Calsequestrin 2 J–L. Gene levels were normalized to GAPDH housekeeping gene and are represented relative to gene level expressed by non-differentiated DCM-C. n = 4–6 of biological replicates. Asterisks denote significant difference by 1-way ANOVA, *p < 0.05, **p < 0.01 ***, p < 0.001 and ****p < 0.0001