Fig. 2

Generation of iPSCs using a chemically defined, integration-free methodology. A Time schedule of fibroblast reprogramming and iPSCs generation. B Phase contrast images of fibroblasts during reprogramming on different days and morphology of establishing DCM-iPS colonies. C Morphology of established iPS Lines. Cells were viewed using a FLoid^® Cell Imaging Station. D Sanger sequencing of the PCR product derived from the genomic DNA of the clones shows the complement sequence of mutation 2156_2157delAG in PLEKHM2 gene in DCM-iPSC lines. Scale bar 100 μm