Fig. 8

EA promoted the expression of srGAP3 and inhibited the expression of Rac1 GTP, p-NR2B, PSD95 and the ratio of F/G in the spinal dorsal horns of rats with SNL, while CN04 reversed the effects of EA on the expression of Rac1 GTP, p-NR2B, PSD95 and the ratio of F/G but had no significant effect on the expression of srGAP3. A–F Representative Western blots and quantitative analysis of srGAP3/GAPDH expression. n = 5. B–G Representative Western blots and quantitative analysis of Rac1-GTP/total Rac1 expression. n = 5. C–H Representative Western blots and quantitative analysis of p-NR2B/NR2B expression. n = 5. D–I Representative Western blots and quantitative analysis of PSD95/GAPDH expression. n = 5. E–J Representative Western blots and quantitative analysis of the F/G ratio. n = 5. K, L Representative photos showing immunohistochemical staining of srGAP3 and active Rac1-GTP cells (The orange arrows show some representative srGAP3- and active Rac1-GTP-positive cells). Scale bars, 20 μm. M, N Number of srGAP3- and active Rac1-GTP-positive cells per field in the spinal dorsal horn. n = 4. ^$$p < 0.01 vs. the SNL group, ^&&p < 0.01 vs the SNL + EA group