Fig. 2

The expression of srGAP3 increased gradually in the initial stage of peripheral nerve injury and decreased gradually in the later stage, while the activity of Rac1 decreased gradually in the initial stage and increased gradually in the later stage after SNL. A, B Representative Western blots and quantitative analysis of srGAP3/GAPDH expression. n = 5. C, D Representative Western blots and quantitative analysis of Rac1-GTP/total Rac1 expression. n = 5. E Representative photos of immunohistochemical staining of srGAP3 and active Rac1-GTP cells (The orange arrows show some representative srGAP3- and active Rac1-GTP-positive cells). Scale bars, 20 μm. F, G Number of srGAP3- and active Rac1-GTP-positive cells per field in the spinal dorsal horn. n = 4. Columns represent the mean ± SD. ^**p < 0.01 vs. the sham group; ^&&p < 0.01 vs the SNL (10) group