Fig. 8

AMPK and ULK1 inhibitors alleviated the autophagy and atrophy of C2C12 myotubes under hypoxia. A–D Western blot results showing total and phosphorylation of AMPKα, ULK1, and mTOR and autophagy- and atrophy-related protein levels in C2C12 myotubes with and without AMPK inhibitor, Dorsomorphin, and ULK1 inhibitor, SBI-0206965, after exposure to hypoxia for 24 h. Statistics were from three independent replicates of the samples and data were expressed as mean ± SEM; the remaining two groups were compared with the hypoxic group, respectively, and P values are the results of one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significant difference. Co: iPSCs and C2C12 co-culture